Ion-exchange Chromatography Protocol

The extracts was kept at -18 ºC or lyophilized Stationary Phase: Anion exchange-DEAE-Sepharose column Eluent: For obtaining neutral fraction

Thus it seems possible to use DEAE-cellulose column chromatography for detec- tion of S + revertants in populations of polycation-stimulated

deae cellulose column chromatography The Application of DEAE-cellulose Column Chromatography to the Selection of S+ Revertants and Determination of Reversion Frequency in Populations of S- Mutants

deae column chromatography A high-performance liquid-chromatographic method based on coupled DEAE anion-exchange and Protein G affinity columns has been developed for the simultaneous